Livestock Studies 2005, Vol 45, Num, 2     (Pages: 001-007)

Different Semen Extenders Effect on Motility of Holstein Bull Semen Stored at +4ºC

Pürhan Barbaros TUNCER 1 ,Mesut ÇEVİK 2 ,Hüseyin KİNET 1

1 Lalahan Hay. Mrk. Araş. Enstitüsü, Sun’i Toh. Lab. – Ankara / TÜRKİYE
2 OMU Vet. Fak. Dölerme ve Sun’i Tohumlama ABD – Samsun / TÜRKİYE
- This study was conducted to compare Laiciphose W 488 and AndroMed extenders for maintaining sperm motility at 4ºC in bulls. In the study 40 ejaculates collected from 4 Holstein bulls were used. Ejaculates were divided into two groups and were diluted with Laiciphos W 488 and AndroMed. Diluted semen was cooled to +4ºC in 45 minutes. After that they were transferred into the refrigerator. Sperm motility was estimated daily up to day 10 post dilution. Immediately after dilution sperm motility rates were 83.37±0.37 % and 83.12±0.38 % for Laiciphos W 488 and AndroMed extenders, respectively. Sperm motility for the samples diluted with AndroMed on day 3 was above 50 % in all bulls studied. However, motility rates in sperm samples diluted with Laiciphos W 488 were higher than 50% in 2 bulls only. Motility rates gradually decreased day by day after day 3 post-dilution and, finally, dropped zero on day 10 in the both extender groups. As a result, it was concluded that bull sperm diluted with both extenders which can be used for artificial insemination during 3 days at +4ºC and no significant difference was determined between extenders on the preservation of sperm motility. Keywords : AndroMed, Bull Semen, Laiciphose 488, Motility