The freezing of ram sperm in liquid nitrogen like pellet by using various methods

After having equilibrated for two hours, they were frozen in liquid nitrogen vapor in pellet form using 3 different technics and were kept in liquid nitrogen first section of the first row of the aluminium cup was left as the some in the first technic, second section was covered with aluminium foil, the third section was covered with plastic sheath. As the cup was on the shelf of freezing tank, the diluted semen which was taken with a syringe was emptied to the section in 0.2 cm3 doses. The pellets which were frozen in liquid nitrogen vapor were kept in liquid nitrogen for 7 minutes.

In the second technic the aluminium cups were prepored as in the first technic and the section of aluminium cups were filled with 0.2 cm3 diluted semen in a frozen cup and the cup was taken to the shelf of the freezing tank after having been rolled with aluminium foil. The pellets were frozen after having been kept in liquid nitrogen vapor and stored in liquid nitrogen.

In the third technic as the aluminium cup was on the shelf of freezing tank, semen in gelatin capsules were put in the holes of the sections. The diluted semen were injected to the gelatine capsules in 0.2 cm3 doses. The capsules which were kept in liquid nitrogen vapor were there kept is liquid nitrogen.

After melting the pellets which were prepared as is the first technic there were no motilite spermatozoa in the microscopical examination, whereas 65 % motile spermatozoa was detected before freezing. After melting the pellets which were prepared as in the second technic there was an avarage of 8 % motile spermatozoa in the microscopical examination whereas the rate of motility was 65 %, before freezing. After melting the pellets which were prepared as in the third technic there was 20 % motile spermatozoa in the microscopical examination whereas the rate of was 65 % before freezing. Keywords :