Blood protein polymorphism and its use in parentage test in dairy cattle breeds
2 Tierarztliche Hochschule, Hannover, GERMANY - The study was conducted to determine the phenotypes and allel frequencies of Hb, Tf, Pa, AmI, AmII and Cp in blood and to check their value in parentage tests. The material of the study consisted of 365 Turkish Brown and 165 Holstein cattle wihch constituted 174 family groups.
Protein variants were determined by the methods of starch - gel and polyacrylamide - gel electrophoresis. The phenotype and allel frequencies were calculated. The distribution of Aml, Amll and Hb genotypes in Holsteins and Aml genotypes in Browns deviated significantly from the expected frequencies (P<0.01). No polymorphism was identified in the ceruloplasmin iocus in both breeds.
Using the ploymorphic systems individually, 2.8 to 12 % pedigree errors were detected. When th systems combined, the rate of false pedigrees were increased to 18.4 %. According to the combined system, the exclusion probability was 65.7 percent.
It was concluded that the effectiveness of parentage tests could be rnaximised by using combined polymorphic system instead of individual systems. It may also be suggested that all the related personnel should be trained to minimise the possibility of pedigree errors.
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