The Studies on In-Vitro Evaluation of Stallion Semen Frozen With Different Extenders

Semen taken from five Arabian Stallions were extended in a way that to ensure 100x10⁶ /cm³ motile spermatozoa by lactose and tris extenders and frozen in the macrotube in liquid nitrogen vapour.

In the semen after thawing of 50ºC-40 seconds the average values of semen; sperm motility (%),total percentage of abnormal sperm (%), percentage of abnormal acrosome (%) and thermo-rezistance test motility (%) in 1., 2. and 3. hours were recorded; %47.44; %31.73; %22.64; %22.74; %9.13 and %2.58 respectively for frozen semen by lactose.

In the semen after thawing of 50ºC-40 seconds the average values of sperm were recorded; %37.83; %36.06; %24.14; %17.58; %5.50 and %0.99 respectively as mentioned above for frozen semen by tris.

In the frozen semen by lactose extender, after thawing of 45ºC-30 seconds spermatolojical charecteristics were recorded; %42.33; %35.52; %24.59; %19.94; %6.74 and %1.13 respectively as mentioned above.

In the diluted semen by tris extender, after thawing of 45ºC-30 seconds spermatolojical charecteristics were recorded; %34.63; %38.39; %25.79; %15.94; %4.96 and %0.83 respectively as mentioned above. As a conclusion, extending stallion semen by lactose was more succesfull compare to by tris extenders and thawing at 50ºC-40 seconds was given better results than thawing at 45ºC-30 seconds. On the other hand, no relation was found semen extenders and thawing temperature and time. Keywords : Stallion, Semen, Dilution, Freezing, Thawing