Livestock Studies 2002, Vol 42, Num, 1     (Pages: 001-009)

Effect of Glucose on In-vitro Capacitation of Bull Spermatozoa

İlker SERİN 1 ,Necmettin TEKİN 2 ,Ergun AKÇAY 2 ,Ahmet CEYLAN 1

1 Adnan Menderes Üniversitesi, Veteriner Fakültesi Doğum ve Reprodüksiyon Hastalıkları AD. -AYDIN
2 Ankara Üniversitesi, Veteriner Fakültesi, Dölerme ve Sun’i Tohumlama AD. -ANKARA
Viewed : 823 - Downloaded : 581 In this study, the effect of glucose on in-vitro capacitation of frozen-thawed bull semen was investigated. For this purpose, sperm were incubated with a dose of 100 μg/ml heparin in the presence (HG) or absence of 5 mM glucose (H) for a 60 min. Heparin induced capacitation was determined by the undergo an acrosome reaction upon exposure to 100 μg/ml lysophosphatidylcholine (LC). To be able to determine the reaction, spermatozoa were stained by Giemsa. The acrosome stained dark pink for the non-reacted sperm and remain unstained for the reacted sperm. Mean proportions of acrosome reacted spermatozoa for the HG and H groups were 20.8% and 43.4% respectively. Consequently, when sperm were incubated under capacitating conditions with heparin plus glucose reduced the LC induced acrosome reactions (p<0.001). Keywords : Bull spermatozoa, spermatozoa capacitation, heparin, glucose